Protective effects of autologous bone marrow-derived mesenchymal stem cell transplantation on acute radioactive enteritis in Beagle dogs

Figure 3 Transplantation and tracing for super paramagnetic iron oxide-labeled autologous bone marrow-derived mesenchymal stem cells in vivo. A: Identification of cultured autologous bone marrow-derived mesenchymal stem cells (ABMSCs) labeled with super paramagnetic iron oxides (SPIOs) via Prussian blue staining and light microscopy (bars, 200 μm); B: The growth curve of ABMSCs labeled with different dose of SPIOs was determined by MTT assay. Each experiment was conducted in triplicate, and the values are reported as the mean ± SD; C: Abdominal aorta and mes-enteric artery branches were checked using femoral artery puncture and digital subtraction angiography imaging; D: Magnetic resonance imaging showed the results of T2 weighted image intensity comparison at the same layer; E: Observation of SPIO and Prussian blue–stained frozen sections via light microscopy. Bars, 100 μm. ^aP < 0.05 vs Blank group vs 30 mg/L group; SPIO: Super paramagnetic iron oxide; ABMSCs: Autologous bone marrow-derived mesenchymal stem cells; MRI: Magnetic resonance imaging.