Adenosine triphosphate-binding pocket inhibitor for mixed lineage kinase domain-like protein attenuated alcoholic liver disease via necroptosis-independent pathway

Figure 3 Necroptosis-induced cell death occurs after demethylation in RAW 264. 7 cell. A: RAW 264.7 cells treated with 5-aza-2’-deoxycytidine (5AD) to determine cell cytotoxicity; B: In HT29 and RAW 264.7 cells, cell viability was tested via demethylation with 5AD for 72 hours and induction of necroptosis for 24 hours; C: After inducing necroptosis in HT29 and RAW 264.7 cells, cell viability was determined using Incucyte assay. 5AD: 5-aza-2’-deoxycytidine.