Keratin 1 modulates intestinal barrier and immune response via kallikrein kinin system in ulcerative colitis

Figure 5 Intestinal changes and alterations in intestinal mechanical barrier marker gene expression in different treatment groups. A: Hematoxylin-eosin staining of intestinal cross-sections from different treatment groups (100 ×); B: Real-time polymerase chain reaction (RT-qPCR) detection results of occludin expression in different groups; C: Western blot results of occludin, zonula occludens-1, claudin, and coagulation factor XII in different groups; D: Grayscale quantification of Western blot data for occludin in different groups; E: RT-qPCR detection results of zonula occludens-1 in different groups; F: Grayscale quantification of Western blot data for zonula occludens-1 in different groups; G: RT-qPCR detection results of claudin expression in different groups; H: Grayscale quantification of Western blot data for claudin in different groups; I: Grayscale quantification of Western blot data for coagulation factor XII in different groups. DSS: Dextran sulfate sodium; ZO-1: Zonula occludens-1; FXIIα: Coagulation factor XII; GAPDH: Glyceraldehyde-3-phosphate dehydrogenase.