Keratin 1 modulates intestinal barrier and immune response via kallikrein kinin system in ulcerative colitis

Figure 3 Effects of different concentrations of the keratin 1 antibody on intestinal mechanical barrier marker genes in Caco-2 cells. A: Real-time polymerase chain reaction (RT-qPCR) results showed a reduction in the expression levels of occludin treated with varying concentrations of the keratin 1 (KRT1) antibody; B: Western blot quantification revealed decreased expression of occludin in Caco-2 cells treated with the KRT1 antibody; C: Western blot analysis of occludin, zonula occludens-1, claudin, and coagulation factor XII in Caco-2 cells under different treatment conditions; D: RT-qPCR results showed a reduction in the expression levels of zonula occludens-1 treated with varying concentrations of the KRT1 antibody; E: Western blot quantification revealed decreased expression of zonula occludens-1 in Caco-2 cells treated with the KRT1 antibody; F: RT-qPCR results showed a reduction in the expression levels of claudin in Caco-2 cells treated with varying concentrations of the KRT1 antibody; G: Western blot quantification revealed decreased expression of claudin in Caco-2 cells treated with the KRT1 antibody; H: Western blot quantification increased expression of coagulation factor XII, in Caco-2 cells treated with the KRT1 antibody. ^cP < 0.001. P value vs control group. Treatment 1: Keratin 1 (KRT1) antibody at 1 ng/mL; Treatment 2: KRT1 antibody at 5 ng/mL; Treatment 3: KRT1 antibody at 10 ng/mL. ZO-1: Zonula occludens-1; FXIIα: Coagulation factor XII; GAPDH: Glyceraldehyde-3-phosphate dehydrogenase.