Keratin 1 modulates intestinal barrier and immune response via kallikrein kinin system in ulcerative colitis

Figure 2 Effect of different concentrations of keratin 1 antibody on the expression levels of high molecular weight kininogen and bradykinin in Caco-2 cells. A: Immunofluorescence analysis shows a reduction in high molecular weight kininogen expression in Caco-2 cells treated with different concentrations of keratin 1 (KRT1) antibody; B: Enzyme-linked immunosorbent assay results demonstrate an increase in bradykinin expression in Caco-2 cells treated with various concentrations of KRT1 antibody. ^aP < 0.05. ^cP < 0.001. P value vs control group. Treatment 1: Keratin 1 (KRT1) antibody at 1 ng/mL; Treatment 2: KRT1 antibody at 5 ng/mL; Treatment 3: KRT1 antibody at 10 ng/mL. ELISA: Enzyme-linked immunosorbent assay.