Histopathological diagnosis of microvascular invasion in hepatocellular carcinoma: Is it reliable?

Figure 1 Schematic flowchart illustrating the process of producing whole-mount slide images. A: The entire liver lobe containing the tumor was sectioned in parallel at a uniform thickness of 3 mm along the maximal tumor section; B: Tissue blocks were consecutively numbered and oriented in the same direction for embedding; C: The first complete whole-mount slide images stained with hematoxylin and eosin was prepared for each tissue block. Whole-mount slide images labeled as number 4 correspond to the maximal tumor section, whereas whole-mount slide images labeled as numbers 3-5 correspond to sections containing the tumor at 3 mm intervals. Whole-mount slide images labeled as numbers 1-7 correspond to sections of the entire liver at 3 mm intervals.