Bletilla striata polysaccharides alleviate metabolic dysfunction-associated steatotic liver disease through enhancing hepatocyte RelA/ HNF1α signaling

Figure 1 High-fat-diet feeding induces hepatic steatosis in mice. Male C57BL/6 mice were fed with normal chow (control) or high-fat-diet (60% fat, 20% carbohydrates, 20% protein) for 0, 4, or 8 weeks (n = 12; 72 mice in total), and their body weights were measured and their fasting serum samples were prepared. A: Body weights; B: Liver weights; C: The levels of serum alanine aminotransferase; D: The levels of serum total cholesterol; E: The levels of serum triglycerides (TG); F: Intrahepatic TG contents; G: Apoptosis index; H: Hematoxylin and eosin, Masson and transferase-mediated deoxyuridine triphosphate-nick end labeling (TUNEL) staining of liver tissue sections; I: Western blot analysis of cleaved caspase-3 and mixed lineage kinase domain-like protein phosphorylation. Data are representative images or expressed as the mean ± SD of each group of mice from at least three separate experiments. ^bP < 0.01. ¹P < 0.01 vs the control group. ²P < 0.01 vs the 0 weeks group. HFD: High-fat-diet; ALT: Alanine aminotransferase; TC: Total cholesterol; TG: Triglycerides; HE: Hematoxylin and eosin; MLKL: Mixed lineage kinase domain-like protein; TUNEL: Transferase-mediated deoxyuridine triphosphate-nick end labeling; wk: Week.