Bletilla striata polysaccharides alleviate metabolic dysfunction-associated steatotic liver disease through enhancing hepatocyte RelA/ HNF1α signaling

doi:10.3748/wjg.v31.i4.93179

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Jan 28, 2025 (publication date) through Jan 8, 2025

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Gastroenterol. Jan 28, 2025; 31(4): 93179
Published online Jan 28, 2025. doi: 10.3748/wjg.v31.i4.93179

Table 3 Primary antibody information

Antibody	Source	Lot number	Manufacturer	Reactivity
ATF4	Rabbit mAb	11815	Cell signaling technology, Danvers, MA, United States	Mouse, human
β-actin	Mouse mAb	Sc-81178	Santa Cruz, Dallas, TX, United States	Mouse, human
CHOP	Mouse mAb	Ab11419	Abcam, Cambridge, MA, United States	Mouse, human
Cleaved caspase-3	Rabbit mAb	9664	Cell signaling technology	Human, mouse
GPX4	Mouse mAb	Sc-166437	Santa Cruz	Mouse, human
GRP78	Rabbit mAb	Ab108615	Abcam	Human, mouse
HNF1α	Mouse mAb	Sc-393668	Santa Cruz	Mouse, human
HRD1	Rabbit pAb	Pa5-76137	Thermo Fisher Scientific, Waltham, MA, United States	Mouse, human
MCAD	Mouse mAb	Sc-365109	Santa Cruz	Mouse, human
MLKL	Rabbit mAb	Pa5-34733	Thermo Fisher Scientific	Mouse, human
MTP	Mouse mAb	Sc-515742	Santa Cruz	Mouse, human
p-MLKL	Rabbit mAb	37333s	Cell signaling technology	Mouse, human
RelA	Mouse mAb	Sc-514451	Santa Cruz	Mouse, human
SREBP1	Mouse mAb	Ma5-16124	Thermo Fisher Scientific	Mouse, human

CHOP: CCAAT-enhancer-binding protein homologous protein; GPX4: Glutathione peroxidase 4; GRP78: 78-kDa glucose-regulated protein; MTP: Microsomal triglyceride transfer protein; HNF1α: Hepatocyte nuclear factor-1 alpha; HRD1: 3-hydroxy-3-methyl glutaryl coenzyme A reductase degradation 1 homolog; MCAD: Medium chain acyl-CoA dehydrogenase; mAb: Monoclonal antibody; MLKL: Mixed lineage kinase domain-like protein; pAb: Polyclonal antibody; p-MLKL: Phosphorylated mixed lineage kinase domain-like protein; SREBP1: Sterol regulatory element-binding protein 1; ATF4: Activating transcription factor 4; RelA: Nuclear factor kappa B p65.

Citation: He YH, Ou LL, Jiang JL, Chen YF, Abudukeremu A, Xue Y, Mu MY, Zhong WW, Xu DL, Meng XY, Guan YQ. Bletilla striata polysaccharides alleviate metabolic dysfunction-associated steatotic liver disease through enhancing hepatocyte RelA/ HNF1α signaling. World J Gastroenterol 2025; 31(4): 93179
URL: https://www.wjgnet.com/1007-9327/full/v31/i4/93179.htm
DOI: https://dx.doi.org/10.3748/wjg.v31.i4.93179