RGS4 promotes the progression of gastric cancer through the focal adhesion kinase/phosphatidyl-inositol-3-kinase/protein kinase B pathway and epithelial-mesenchymal transition

Figure 3 Overexpression of regulator of G protein signaling 4 promoted the proliferation, migration, and invasion of gastric cancer cells in vitro. A: The interference efficiency of the lentiviral carrying regulator of G protein signaling 4 (RGS4) was validated by real-time quantitative polymerase chain reaction (left) and western blot (right) analysis (overexpressing RGS4); B: The proliferation of AGS cells with RGS4 overexpression was determined by cell counting kit-8 assays; C: The effects of RGS4 overexpression on the colony formation of AGS cells; D: The migration ability of AGS cells was measured by wound healing; E: The invasion of AGS cells with RGS4 overexpression was determined by transwell assays. ^aP < 0.05, ^bP < 0.01, ^dP < 0.0001, NS: No significance. RGS4: Regulator of G protein signaling 4.