RGS4 promotes the progression of gastric cancer through the focal adhesion kinase/phosphatidyl-inositol-3-kinase/protein kinase B pathway and epithelial-mesenchymal transition

Figure 2 Regulator of G protein signaling 4 knockdown inhibited the proliferation, migration, and invasion of gastric cancer cells in vitro. A: The expression levels of regulator of G protein signaling 4 (RGS4) in different gastric cancer (GC) cell lines were detected by real-time quantitative polymerase chain reaction; B: The interference efficiency of the lentiviral human RGS4-targeting short hairpin RNA was validated by real-time quantitative polymerase chain reaction (left) and western blot (right) analysis; C: The proliferation of MGC-803 cells with RGS4 knockdown was determined by cell counting kit-8 assays; D: The effects of RGS4 knockdown on the colony formation of MGC-803 cells; E: The migration ability of MGC-803 cells was measured by wound healing; F: The invasion of MGC-803 cells with RGS4 knockdown was determined by transwell assays. ^aP < 0.05, ^bP < 0.01, ^cP < 0.001, NS: No significance. RGS4: Regulator of G protein signaling 4.