Bicuculline ameliorates metabolic dysfunction-associated steatotic liver disease by inhibiting the nuclear factor-kappa B pathway and reducing lipid accumulation

Figure 5 Bicuculline affects lipid accumulation in free fatty acid-treated AML12 cells through the nuclear factor-kappa B pathway. A: After immunofluorescence staining, the localization of nuclear factor-kappa B p65 (green) was observed using confocal microscopy. The nuclei were stained with DAPI (blue); B and C: Representative western blotting results and density analysis of p65 and p50 expression; the results are expressed as the means ± SD (n = 2); D: QRT-PCR analysis of Dgat1, Dgat2, Scd1, Srebf1, Fasn, IL-1β, COX2, and TNF-α gene expression. The results are expressed as the mean ± SD (n = 4). Compared with the model group. ^aP < 0.05; ^bP < 0.01; ^cP < 0.001; BIC: Bicuculline.