Bicuculline ameliorates metabolic dysfunction-associated steatotic liver disease by inhibiting the nuclear factor-kappa B pathway and reducing lipid accumulation

Figure 3 The therapeutic effect of bicuculline in a high-fat model of isolated hepatocytes. A and B: Cytotoxic effects of different concentrations of bicuculline on AML12 cells and HepG2 cells; C: Oil red O staining results under light microscopy (200 ×); D-G: Relative total triglyceride and cholesterol levels in the two cell types; H-K: Absolute enzyme activity of alanine aminotransferase and aspartate aminotransferase in the two cell types. The results are expressed as the means ± SDs (n = 5). Comparison between the treatment group and model group. ^aP < 0.05; ^bP < 0.01; TC: Total cholesterol; ALT: Alanine aminotransferase; AST: Aspartate aminotransferase; TG: Triglyceride.