Bicuculline ameliorates metabolic dysfunction-associated steatotic liver disease by inhibiting the nuclear factor-kappa B pathway and reducing lipid accumulation

Figure 2 Evaluation of the therapeutic effect of bicuculline on metabolic dysfunction-associated steatotic liver disease in zebrafish larvae. A: Hematoxylin & eosin staining of the livers of zebrafish larvae in each group; B: Whole-fish oil red O staining; C: Effects of bicuculline on lipid metabolism-related genes and inflammatory factors. The results are expressed as the means ± SDs (n = 5). One-way ANOVA with Tukey’s post hoc test was used to compare all the groups. Compared with the model group, ^aP < 0.05; ^bP < 0.01; ^cP < 0.001; MASLD: Metabolic dysfunction-associated steatotic liver disease; BIC: Bicuculline; H&E: Hematoxylin & eosin; NC: Negative control.