Interventional effect of hesperetin on N-methyl-N’-nitro-N-nitrosoguanidine-induced exosomal circ008274 in affecting normal cells to promote gastric carcinogenesis

Figure 6 Hesperetin mediated N-methyl-N’-nitro-N-nitrosoguanidine induced gastric cancer by targeting miR-526b-5p through exosomal circ0008274. A: Circlnteractome and circ-atlas databases showed that Argonaute 2 has the ability to bind to circ0008274; B: Circ-bank and circ-atlas databases predicted the microRNAs (miRNAs) that circ0008274 binds to and take the intersections; C: MiR-526b-5p was highly expressed and miR-4676-5p was lowly expressed after circ0008274 knockdown; D: MiR-526b-5p was lowly expressed after overexpression of circ0008274; E: Dual-luciferase reporter gene assay; F: Quantitative polymerase chain reaction detection of miR-526b-5p expression in cells and exosomes; G: MiR-526b-5p expression after transfection of miR-526b-5p mimics/inhibitor; H: Circ0008274 expression after transfection of miR-526b-5p mimics/inhibitor; I: Proliferative capacity of TGES-1 cells after treated with exosomes from different groups; J: Changes in clonogenic capacity of TGES-1 cells treated with exosomes from different groups; K: Changes in migration and invasion capacity of TGES-1 cells treated with exosomes from different groups. ^aP < 0.05. ^bP < 0.01. ^cP < 0.001. ^dP < 0.0001. EX: Exosome; LV: Lentivirus vector; MUT: Mutant; NC: Negative control; OD: Optical density; OE: Overexpression; si-NC: Small interfering RNA negative control; TGES-1-EX: Exosomes derived from TGES-1 cells; WT: Wild-type.