Interventional effect of hesperetin on N-methyl-N’-nitro-N-nitrosoguanidine-induced exosomal circ008274 in affecting normal cells to promote gastric carcinogenesis

Figure 5 Hesperetin pretreatment of exosomes from overexpressed/knockdown circ0008274 TGES-1 cells action on GES-1 cells. A: Expression level of circ0008274 after transfection with overexpressing lentivirus; B: Level of circ0008274 in TGES-1 cells after puromycin screening; C: Expression of circ0008274 after TGES-1 transfection with small interfering RNA; D: Circ0008274 expression in exosomes of hesperetin pretreated overexpressing/knockdown circ0008274 TGES-1 cells; E: Circ0008274 expression after hesperetin pretreatment overexpression/knockdown of exosomes of circ0008274 TGES-1 cells acting on GES-1 cells; F: Changes in growth capacity of GES-1 cells treated with exosomes from different groups; G: Protein levels of epithelial-to-mesenchymal transition (EMT), stemness and proliferation markers in GES-1 cells treated with hesperetin and overexpression/knockdown circ0008274 exosomes derived from TGES-1 cells (TGES-1-EX); H: Changes in clonogenic capacity of GES-1 cells treated with exosomes from different groups; I: Changes in migration and invasion capacity of GES-1 cells treated with exosomes from different groups; J: mRNA expression of EMT, stemness and proliferation markers in GES-1 cells treated with hesperetin and overexpression/knockdown circ0008274 TGES-1-EX. ^aP < 0.05. ^bP < 0.01. ^cP < 0.001. ^dP < 0.0001. GAPDH: Glyceraldehyde-3-phosphate dehydrogenase; LV: Lentivirus vector; NC: Negative control; OD: Optical density; OE: Overexpression; si-NC: Small interfering RNA negative control; si-NC-EX: Small interfering RNA negative control-exosome; TGES-1-EX: Exosomes derived from TGES-1 cells.