Interventional effect of hesperetin on N-methyl-N’-nitro-N-nitrosoguanidine-induced exosomal circ008274 in affecting normal cells to promote gastric carcinogenesis

Figure 4 Identification of circ0008274 and its expression in cells and exosomes. A: Differently expressed circular RNAs (circRNAs) overlapping in public databases; B: Hierarchical clustering analysis of differentially expressed circRNAs as visualized in the heat map; C: Circ0008274 cyclisation mechanism; D: Cellular RNA and exosomes RNA quantitative polymerase chain reaction (qPCR) melting curves; E: Agarose gel electrophoresis; F: qPCR to detect before and after treatment with RNase R circ0008274 expression level; G: Nucleoplasmic separation to determine circ0008274 localization; H: Effect of hesperetin on the level of circ0008274 in cells; I: Modulatory effects of hesperetin on circ0008274 expression in exosomes; J: Expression of circ0008274 in exosomes derived from TGES-1 cells and hesperetin-pretreated TGES-1 cell exosomes after action on GES-1 cells. ^aP < 0.05. ^bP < 0.01. ^cP < 0.001. ^dP < 0.0001. cDNA: Complementary DNA; GAPDH: Glyceraldehyde-3-phosphate dehydrogenase; gDNA: Genomic DNA; TGES-1-EX: Exosomes derived from TGES-1 cells.