LncRNA FTX promotes colorectal cancer radioresistance through disturbing redox balance and inhibiting ferroptosis via miR-625-5p/SCL7A11 axis

Figure 5 SLC7A11 overexpression reverses the effects of radioresistant cells knockdown lncRNA FTX in sensitivity of ferroptosis and radiotherapy. A: Downregulation of SLC7A11 promoted radio-sensitivity in HT29R and HCT116R cells; B: Downregulation of SLC7A11 reduced glutathione (GSH) content in HT29R and HCT116R cells; C: CCK-8 assay revealed that SLC7A11 overexpression increased the tolerance of HT29R-shFTX and HCT116R-shFTX cells to ionizing radiation; D: Colony formation assay confirmed the promoting effect of overexpression of SLC7A11 on the growth of HT29R-shFTX and HCT116R-shFTXcells; E: Western blotting analyses determined the expression of ferroptosis-related proteins in HT29R-shFTX and HCT116R-shFTX cells after SLC7A11 over expression; F: Reversal effect GSH content in HT29R-shFTX, HCT116R-shFTX cells after overexpression of SLC7A11; G: Immunofluorescence analyses revealed overexpression of SLC7A11 and upregulated ROS levels in HT29R-shFTX and HCT116R-shFTX cells. ^aP < 0.05 and ^bP < 0.01. NC: Negative control.