LncRNA FTX promotes colorectal cancer radioresistance through disturbing redox balance and inhibiting ferroptosis via miR-625-5p/SCL7A11 axis

Figure 1 LncRNA FTX is upregulated in colorectal cancer cells and associated with the radiosensitivity of colorectal cancer. A: lncRNA FTX expression in colon, rectal cancer, and para-cancerous tissues; B: Relative expression of lncRNA FTX in HT29 and HCT116 cells after irradiation at different doses; C: LncRNA FTX expression was significantly higher in colorectal cancer radiotherapy-resistant cells than in the corresponding parental cells; D: Knockdown efficiency of lncRNA FTX in HT29R and HCT116R cells was validated by real-time quantitative PCR; E: Knockdown of FTX expression in HT29R and HCT116R markedly increased the sensitivity of cancer cells to radiotherapy; F: Colony formation assay revealed that knockdown of FTX significantly increased the sensitivity of HT29R and HCT116R cells to radiotherapy; G: Cell viability was determined using CCK-8 assay after transfection of sh-FTX and sh-negative control in HT29R or HCT116R cells, which were irradiated by 4 Gy. All representative data are from three independent experiments, and the results are presented as deviation mean ± SD. Statistical analysis was conducted using the student's t-test. ^aP < 0.05 and ^bP < 0.01. NC: Negative control.