Strain- and sex-dependent variability in hepatic microcirculation and liver function in mice

Figure 4 Hematoxylin and eosin staining and immunolabeling analysis of liver tissue from different sexes across BALB/c, C57BL/6J, and KM mice. A: Hematoxylin and eosin (HE) and immunohistochemical staining of estrogen receptor α (ERα), and estrogen receptor β (ERβ), and cluster of differentiation (CD) 31 in the liver. The scale bar represents 50 μm. The insert in the lower panel provides a detailed representation of liver tissues at a higher magnification. Scale bar = 10 μm; B: HE staining of liver tissue sections revealing morphological details. Scale bar = 200 μm. The insert in the lower panel provides a detailed representation of liver tissues at a higher magnification. Scale bar = 50 μm; C: Quantitative analysis of the protein expression levels of ERα, ERβ, and CD31 and the microvascular density in the livers of BALB/c, C57BL/6J, and KM mice. For each group, n = 6 samples, and six microscopic fields of view were selected for each sample. The data are presented as the mean ± SEM. n = 6 each group. ^aP < 0.05. ^bP < 0.01. NS: Not significant; Erα: Estrogen receptor α; Erβ: Estrogen receptor β; CD31: Cluster of differentiation 31.