Long noncoding RNA semaphorin 6A-antisense RNA 1 reduces hepatocellular carcinoma by promoting semaphorin 6A mRNA degradation

Figure 3 Inhibition of semaphorin 6A-antisense RNA 1 promotes proliferation, migration and invasion of hepatocellular carcinoma cells. A and B: Proliferation of Hep3B and LM3 cells stably transfected with semaphorin 6A-antisense RNA 1 (SEMA6A-AS1) short hairpin RNAs (shRNA) or control shRNA were analyzed by Cell Counting Kit-8 assay (A) and colony formation assay (B); C and D: Transwell assay with or without Matrigel were visualized (left panel) and quantified (right panel) in SEMA6A-AS1 shRNA or control shRNA stably transfected LM3 (C) and Hep3B (D) cell lines to analyze cell invasion or migration capability; E: Wound healing assays in SEMA6A-AS1 shRNA or control shRNA stably transfected LM3 and Hep3B cell lines were visualized (left panel) and quantified (right panel) to assess cell migration ability. ^aP < 0.05, ^bP < 0.01, ^cP < 0.001. SEMA6A-AS1: Semaphorin 6A-antisense RNA 1; shRNA: Short hairpin RNAs.