Long noncoding RNA semaphorin 6A-antisense RNA 1 reduces hepatocellular carcinoma by promoting semaphorin 6A mRNA degradation

Figure 2 Overexpression of semaphorin 6A-antisense RNA 1 inhibits proliferation, migration and invasion of hepatocellular carcinoma cells. A and B: Cell proliferation in Hep3B and LM3 cells stably transfected with pc-semaphorin 6A-antisense RNA 1 (pc-SEMA6A-AS1) or pcDNA3.1 plasmid were analyzed by Cell Counting Kit-8 assay (A) and colony formation assay (B); C and D: Transwell assay with or without Matrigel were visualized (left panel) and quantified (right panel) in pc-SEMA6A-AS1 or pcDNA3.1 plasmid stably transfected LM3 (C) and Hep3B (D) cell lines to analyze cell invasion or migration capability; E: Wound healing assays in pc-SEMA6A-AS1 or pcDNA3.1 plasmid stably transfected LM3 and Hep3B cell lines were visualized (left panel) and quantified (right panel) to assess cell migration ability. ^aP < 0.05, ^bP < 0.01, ^cP < 0.001. SEMA6A-AS1: Semaphorin 6A-antisense RNA 1.