Suppressor of cytokine signaling 2 modulates regulatory T cell activity to suppress liver hepatocellular carcinoma growth and metastasis

Figure 7 Suppressor of cytokine signaling 2 suppressing regulatory T-cell cell activity restrains the growth and metastasis of liver hepatocellular carcinoma. A: Morphological images of tumor tissues from different groups of mice and statistical analysis of tumor tissue volume and mass; B: Flow cytometry analysis of regulatory T-cell, Th1and Th17 cell activation in mouse tumor tissues; C: Enzyme-linked immunosorbent assay measuring the expression levels of inflammatory factors in mouse tumor tissues; D: Hematoxylin and eosin (H&E) staining to examine the histology of tumor tissues; E: Immunohistochemical analysis of Foxp3 expression in mouse tumor tissues; F: TUNEL assay to determine the apoptosis rate in mouse tumor tissues; G: H&E staining to identify lung metastatic nodules formed by HepG2 cells. Data are presented as mean ± SD (n = 6; ^aP < 0.05, ^bP < 0.01, ^cP < 0.001, analyzed using analysis of variance and Tukey multiple comparison test). SOCS2: Suppressor of cytokine signaling 2; Treg: Regulatory T-cell; TNF: Tumor necrosis factor; IL: Interleukin.