Decreased gene expression of interleukin 2 receptor subunit γ (CD132) in tissues of patients with Crohn’s disease

Figure 4 Frequency of T cells subset in tissues (flow cytometry). A and B: Comparison of αβ T cells (A) and γδT cells (B) in tissues from patients with Crohn's disease (CD) (n = 7) and healthy controls (n = 7). Values are expressed as percentages, and double T bars indicate standard deviation (^bP < 0.01). The Mann-Whitney U test was used; C: Relationship of interleukin 7 (IL-7) titers in peripheral blood with γδ T cells in tissues of patients with CD; D-G: Spearman's r test was used. Representative figures of αβ and γδ T cell subsets in tissues from healthy controls (D and E) and patients with CD (F and G) vs T cells were gated based on their low side scatter and high cluster of differentiation 45 (CD45) expression. After selection, we differentiated αβ+ (Panel D and F) and γδ+ (Panel E and G) T cell subsets in CD3 vs αβ/γδ dot plot.