Decreased gene expression of interleukin 2 receptor subunit γ (CD132) in tissues of patients with Crohn’s disease

Figure 3 Caspase-3 protein in tissues (Western blot analysis). A: Differences of caspase 3 protein [arbitrary units (AU)] in tissues of patients with Crohn's disease (CD) (n = 24) vs healthy controls (n = 24). Values are expressed as means (× 10⁶/mL), and double T bars denote standard deviation (^cP < 0.001). Mann-Whitney U test was used; B: Interleukin 7 (IL-7) protein expression. Western blot analysis was performed with protein extracts from intestinal biopsies. Loading control was done with anti-actin, 1:1000) (not shown). The visualized fragments were quantified by densitometry using ImageJ software (National Institutes of Health, Bethesda, MD, United States). Quantification of the expression band performed by ImageJ software; C: Significant relationship between γδ T cells subsets in peripheral blood and caspase-3 in tissues of healthy subjects. Spearman’s r test was used; D: Relationship between caspase-3 protein in tissue and IL-7 gene expression and Il-7 protein in tissues of patients with CD (n = 25). Spearman’s r test was used.