Increased keratin 80 expression predicts poor prognosis and promotes oxaliplatin resistance in gastric cancer

Figure 6 Elongation factor 1-alpha 1 interacts with and increases keratin 80 stability in gastric cancer cells. A: The mRNA expression levels of selected genes were quantified by quantitative real-time PCR (qRT-PCR); B: Western blot analysis of the interaction between keratin 80 (KRT80) and elongation factor 1-alpha 1 (EEF1A1) by co-immunoprecipitation; C: The expression of KRT80 and EEF1A1 was analyzed by qRT-PCR in gastric cancer tissues and adjacent normal tissues; D: Correlation analysis revealed a positive relationship between the expression levels of KRT80 and EEF1A1 in tumor samples; E: Western blot analysis of KRT80, EEF1A1, p-P65 and pIκB expression in KRT80-overexpressing cells, with or without EEF1A1 knockdown by siRNA transfection; F: KRT80-overexpressing SGC7901 cells, with or without EEF1A1 knockdown, were treated with 10 μM oxaliplatin and 20 μg/mL cycloheximide (CHX) for the indicated time points. KRT80 protein levels were then assessed by Western blot. Data are shown as the mean ± SD (n = 3). ^aP < 0.05; ^bP < 0.05; ^cP < 0.001; KRT80: Keratin 80; EEF1A1: Elongation factor 1-alpha 1.