Characterization of hepatic pathology during azoxymethane-induced acute liver failure

Figure 7 Hepatocellular senescence is increased in response to azoxymethane toxicity. A: Representative Sudan Black B histochemistry images in liver from vehicle and azoxymethane (AOM)- treated mice at the pre, minor, major and coma time points; B: Liver Sudan Black B staining in mice treated with vehicle or AOM at pre, minor, major and coma time points reported in percent area; C and D: Representative immunoblot and quantification of relative p21 protein from liver homogenates of vehicle- and AOM-treated mice at the pre, minor, major and coma time points. β-actin was used as a protein loading control; E: Representative immunohistochemistry images stained for p21 in livers from vehicle- and AOM-treated mice at the pre, minor, major and coma time points. n ≥ 3 for each group for all analyses. ^aP < 0.05 compared to vehicle-treated mice.