Characterization of hepatic pathology during azoxymethane-induced acute liver failure

Figure 3 Azoxymethane-induced liver injury is associated with increased oxidative stress. A: Hepatic GSH concentration expressed as µmol per gram of liver tissue in mice treated with vehicle or azoxymethane (AOM) at the pre, minor, major and coma time points; B: Liver MDA concentration in µmol normalized to total protein concentrations in mice treated with vehicle or AOM at the pre, minor, major and coma time points; C: H₂O₂ concentration in nmol per gram of liver tissue in mice treated with vehicle or AOM at the pre, minor, major and coma time points; D: Hepatic MPO activity reported as nmol of MPO per minute per mg of protein in mice treated with vehicle or AOM at the pre, minor, major and coma time points; E and F: Representative immunoblot and quantification of immunoblots for SOD1 in liver homogenates from mice administered vehicle or AOM at the pre, minor, major and coma time points. GAPDH is used as a protein loading control. n ≥ 3 for each group for all analyses. ^aP < 0.05 compared to vehicle-treated mice.