scRNA-seq of the intestine reveals the key role of mast cells in early gut dysfunction associated with acute pancreatitis

Figure 9 CCL5 derived from mast cells promotes infiltration of neutrophils and macrophages. A: Violin plots displaying the expression of CCL5 across each group in the small intestine; B: CCL5 mRNA expression levels in the small intestine of the AP1, AP2, and normal groups; C: Flow cytometry analysis of CCL5 expression in CD117-positive cells in the small intestine; D: CCL5 mRNA expression levels in MC/9 cells treated with varying amounts of primary acini; E: Identification of genotypes in c-Kit^W-sh/W-sh mice; F: Weight comparison between c-Kit^W-sh/W-sh mice and wild-type mice; G: Workflow schematic for mouse experiments; H: Immunofluorescence staining confirms the successful inoculation of MC/9 cells into the small intestine; I: HE staining of the pancreas in c-Kit^W-sh/W-sh mice inoculated with MC/9 cells (left) and semiquantitative analysis (right); J: Amylase and lipase activity in plasma from c-Kit^W-sh/W-sh mice inoculated with MC/9 cells (CCL5 shRNA or control); K: HE staining of the intestine of c-Kit^W-sh/W-sh mice inoculated with MC/9 cells (CCL5 shRNA or control); L: 16S rDNA expression in plasma from c-Kit^W-sh/W-sh mice inoculated with MC/9 cells (CCL5 shRNA or control); M: Immunofluorescence staining of CD68 in the small intestine of c-Kit^W-sh/W-sh mice inoculated with MC/9 cells (CCL5 shRNA or control); N: Immunofluorescence staining of Ly6G in the small intestine of c-Kit^W-sh/W-sh mice inoculated with MC/9 cells (CCL5 shRNA or control). MC: Mast cell; NS: No significance. ^aP < 0.001; ^bP < 0.05; ^cP < 0.01.