scRNA-seq of the intestine reveals the key role of mast cells in early gut dysfunction associated with acute pancreatitis

Figure 8 Stabilization of mast cells alleviates intestinal damage and inflammation in the intestine of acute pancreatitis. A: Workflow schematic for mouse experiments; B: Representative images of toluidine blue staining of small intestine samples from the Cromolyn sodium-treated and control groups (left) and semiquantitative analysis of activated mast cells (right); C: Relative levels of 16S rDNA in plasma from the Cromolyn sodium-treated and control groups; D: HE staining of the small intestine in the Cromolyn sodium-treated and control groups; E: Gene targeting strategy for Rosa26-CAG-LSL-tdTomato mice; F: Genotype identification of Rosa26-CAG-LSL-tdTomato mice; G: Workflow schematic for mouse experiments of Villin-tdT mice; H: Immunofluorescence images of the small intestine in the Cromolyn sodium-treated and control groups; I and J: mRNA expression levels of IL-1β and IL-6 in the small intestine of the Cromolyn sodium-treated and control groups; K: CD68 staining of the small intestine in the Cromolyn sodium-treated and control groups (left) and semiquantitative analysis (right); L: Ly6G staining of the small intestine in the Cromolyn sodium-treated and control groups (left) and semiquantitative analysis (right). ^aP < 0.001; ^bP < 0.05; ^cP < 0.01.