NMDAR2B/PKA/CREB signaling pathway contributes to esophageal neuropathic pain in gastroesophageal reflux disease

Figure 8 Results of Western blot of substance P and calcitonin gene-related peptide in the distal esophageal mucosa of rats in each group. A: Relative expression of substance P and calcitonin gene-related peptide in the distal esophageal mucosa among different groups of rats. Data are presented as mean ± SD; B: Relative expression of N-methyl-D-aspartate (NMDA) pathway proteins in the distal esophageal mucosa of rats in each group [1: Model; 2: Control; 3: Model + NMDA receptor (NMDAR) agonist; 4: Model + NMDAR antagonist; 5: Model + protein kinase A (PKA) antagonist; 6: Model + NMDAR antagonist + PKA agonist]. ^aP < 0.001, model group vs control group. ^bP < 0.01, ^cP < 0.001, model + NR agonist group, model + NR antagonist group, model + PKA antagonist group vs model group. ^dP < 0.01, model + NR antagonist + PKA agonist group vs model + NR antagonist group. NR: N-methyl-D-aspartate receptor; PKA: Protein kinase A; SP: Substance P; CGRP: Calcitonin gene-related peptide.