Trichostatin A augments cell migration and epithelial-mesenchymal transition in esophageal squamous cell carcinoma through BRD4/c-Myc endoplasmic reticulum-stress pathway

Figure 4 Trichostatin A promotes epithelial-mesenchymal transition of esophageal squamous cell carcinoma cells through cellular myelocytomatosis oncogene mediated endoplasmic reticulum stress may be associated with bromodomain-containing protein. A: Western blot shows after trichostatin A (TSA) treatment that bromodomain-containing protein (BRD4) level increased; B: Relative protein level of BRD4 in control cells and BRD4-knockdown cells treated with TSA was examined by western blot; C: Representative images of the migration of Eca109 and EC9706 cells treated with TSA combined with carboxylic acid from Transwell assays; D: Representative images of the migration of Eca109 and EC9706 cells treated with TSA combined with knocking down BRD4 from Transwell assays; E: Western blot shows TSA treated of knock down BRD4 esophageal squamous cell carcinoma cells that epithelial-mesenchymal transition marker E-cadherin level increased, vimentin, β-catenin level decreased. Cell counts are for the corresponding assays of at least five random microscope fields (100 magnification). n = 5, P vs control. ^aP < 0.05. ^bP < 0.01. Bar is 50 μm. TSA: Trichostatin A; JQ-1: Carboxylic acid; GRP78: Glucose-regulated protein 78; BRD4: Bromodomain-containing protein.