Trichostatin A augments cell migration and epithelial-mesenchymal transition in esophageal squamous cell carcinoma through BRD4/c-Myc endoplasmic reticulum-stress pathway

Figure 1 Trichostatin A promotes migration of esophageal squamous cell carcinoma cells by promoting epithelial-mesenchymal transition. A: Representative images of the migration of Eca109 and EC9706 cells treated with histone deacetylase inhibitor trichostatin A (TSA) from Transwell assays; B: TSA enhanced cell migration in Transwell migration assay in dose-dependent manners; C: Changes in Eca109 and EC9706 cell morphology after TSA treatment; D and E: Western blot shows after TSA treatment that acetylated histones H3 and acetylated histones H4 levels increased and epithelial-mesenchymal transition marker E-cadherin level decreased, vimentin, β-catenin levels increased. Cell counts are for the corresponding assays of at least five random microscope fields (100 magnification). n = 5, P vs control. ^aP < 0.05. ^bP < 0.01. Panel A Bar is 50 μm, panel B Bar is 10 μm. TSA: Trichostatin A; acH3: Acetylated histones H3; acH4: Acetylated histones H4.