Inhibition of the SERPINB5/HSP90AA1 axis restrains the proliferation and invasion of rectal cancer

Figure 5 Heat shock protein 90 alpha class A member 1 overexpression reversed the effects of serpin family B member 5 knockdown on SW480 cell progression. A and B: Western blot analysis was utilized to measure heat shock protein 90 alpha class A member 1 protein expression; C: SW480 cell proliferation was evaluated with Cell Counting Kit-8 assay; D and E: SW480 cell invasion was evaluated with Transwell assay; F and G: SW480 cell apoptosis was tested with flow cytometry. Data are presented as mean ± SEM. ^aP < 0.01. HSP90AA1: Heat shock protein 90 alpha class A member 1; GAPDH: Glyceraldehyde-3-phosphate dehydrogenase; sh: short-hairpin RNA; SERPINB5: Serpin family B member 5; FITC: Fluorescein isothiocyanate; PI: Propidium iodide.