Inhibition of the SERPINB5/HSP90AA1 axis restrains the proliferation and invasion of rectal cancer

Figure 4 Effects of heat shock protein 90 alpha class A member 1 on SW480 cell proliferation, invasion, and apoptosis. PcDNA-heat shock protein 90 alpha class A member 1 (HSP90AA1) and short-hairpin RNA targeting HSP90AA1 were transfected into SW480 cells. A and B: HSP90AA1 protein level in SW480 cells was tested through western blot analysis; C: SW480 cell proliferation was evaluated through Cell Counting Kit-8 assay; D and E: SW480 cell invasion was detected with Transwell assay; F and G: SW480 cell apoptosis was tested with flow cytometry. Data are presented as mean ± SEM. ^aP < 0.01. HSP90AA1: Heat shock protein 90 alpha class A member 1; GAPDH: Glyceraldehyde-3-phosphate dehydrogenase; sh: short-hairpin RNA; FITC: Fluorescein isothiocyanate; PI: Propidium iodide.