Inhibition of the SERPINB5/HSP90AA1 axis restrains the proliferation and invasion of rectal cancer

Figure 3 Serpin family B member 5 interacted with heat shock protein 90 alpha class A member 1 in SW480 cells. A: Potential interacting proteins of serpin family B member 5 (SERPINB5) were predicted by using the STRING tool (https://cn.string-db.org/); B and C: Co-immunoprecipitation assay verified that SERPINB5 interacted with heat shock protein 90 alpha class A member 1 (HSP90AA1); D: PcDNA-SERPINB5 and short-hairpin RNA targeting SERPINB5 were transfected into SW480 cells. HSP90AA1 protein level was tested through western blot analysis; E: Gene Expression Profiling Interactive Analysis database revealed the relative expression of HSP90AA1 in rectal cancer tissues (left red) and normal tissues (right gray), ^aP < 0.01; F: Western blot analysis was employed to measure the relative HSP90AA1 protein level in rectal cancer tissues and matched adjacent tissues (n = 27). Data are presented as mean ± SEM. ^aP < 0.01. IP: Immunoprecipitation; IgG: Immunoglobulin G; SERPINB5: Serpin family B member 5; GAPDH: Glyceraldehyde-3-phosphate dehydrogenase; HSP90AA1: Heat shock protein 90 alpha class A member 1; sh: short-hairpin RNA.