Inhibition of the SERPINB5/HSP90AA1 axis restrains the proliferation and invasion of rectal cancer

Figure 2 Effects of serpin family B member 5 on SW480 cell proliferation, invasion, and apoptosis. SW480 cells were transfected with pcDNA-serpin family B member 5 (SERPINB5) or short-hairpin RNA targeting SERPINB5. A and B: SERPINB5 protein level in SW480 cells was measured through western blot analysis; C: SW480 cell proliferation was examined with Cell Counting Kit-8 assay; D and E: SW480 cell invasion was evaluated with Transwell assay; F and G: SW480 cell apoptosis was tested through flow cytometry. Data are presented as mean ± SEM. ^aP < 0.01. SERPINB5: Serpin family B member 5; GAPDH: Glyceraldehyde-3-phosphate dehydrogenase; sh: short-hairpin RNA; FITC: Fluorescein isothiocyanate; PI: Propidium iodide.