NAD+/SIRT1 pathway regulates glycolysis to promote oxaliplatin resistance in colorectal cancer

Figure 5 Increased glycolysis in Oxa-R colorectal cancer cells. A: Enrichment analyses of the Kyoto Encyclopedia of Genes and Genomes pathways associated with resistance-related differentially expressed genes; B: Western blots to determine expression of the key glycolytic enzymes LDHA and PKM2 in HCT116-WT and HCT116 Oxa-R cells. Each bar represents means ± SD of three separate experiments. ^aP < 0.05. β-Actin was used as the internal reference; C: HCT116-WT and HCT116 Oxa-R cells were seeded in 96-well Seahorse assay plates, and glucose (100 mmol/L), oligomycin (10 μM) and 2-DG (500 mmol/L) were successively added to measure the extracellular acidification rate.