NAD+/SIRT1 pathway regulates glycolysis to promote oxaliplatin resistance in colorectal cancer

Figure 1 Screening of key molecules that regulate oxaliplatin resistance in colorectal cancer. A: Differentially expressed genes between HCT116-WT and HCT116 Oxa-R cells are shown in a volcano plot. In this plot, red dots represent upregulated differentially expressed genes, whereas green dots represent downregulated differentially expressed genes; B: Based on the expression profile of resistance-related differentially expressed genes, the Library of Integrated Network-based Cellular Signatures database was queried to identify small molecules with connectivity scores that are positively correlated with the resistance profile. These candidate drugs likely target mechanisms or pathways involved in regulating oxaliplatin resistance; C: The expression of SIRT1 mRNA were associated with overall survival in colorectal cancer patients with p53 mutations; D: Western blotting was performed to detect the expression levels of SIRT1 in both HCT116-WT cells and HCT116 Oxa-R cells. Each bar represents means ± SD of three separate experiments. ^bP < 0.01.