Circular RNA contributes to gastric cancer by targeting Wnt family member 2B as a competing endogenous RNA

Figure 6 Hsa_circRNA_102415 enhances gastric cancer malignancy via the microRNA-4529-5p/WNT2B axis. Transfect small interfering-WNT2B and microRNA (miR)-4529-5p inhibitors into AGS cells for 24 hours. A: Studied and analyzed the transfected AGS cells, and detected the expression of miR-4529-5p using quantitative real-time polymerase chain reaction (RT-qPCR); B: Studied and analyzed the transfected AGS cells, and detect the protein expression of WNT2B using Western blotting; C: Studied and analyzed the transfected AGS cells, and detect the cell proliferation level using cell counting kit-8 method; D and E: Conducted research and analysis on transfected AGS cells, and used Transwell assay to detect the level of cell invasion; F: Study and analyze the transfected AGS cells, and use flow cytometry to detect the proportion of cell cycle stages; G and H: Study and analyze the transfected AGS cells, and detect the level of cell apoptosis using flow cytometry. Data were reported using mean ± SEM. ^bP < 0.01. circRNA: Circular RNA; miR: MicroRNA; NC: Negative control; si-NC: Small interfering negative control; pcDNA: Plasmid DNA; RT-qPCR: Quantitative real-time polymerase chain reaction; FITC: Fluorescein isothiocyanate.