Circular RNA contributes to gastric cancer by targeting Wnt family member 2B as a competing endogenous RNA

Figure 5 MicroRNA-4529-5p targets WNT2B in gastric cancer cells. A: The binding sites of microRNA (miR)-4529-3p and WNT2B were predicted using the Targeted scan database; B: The relative luciferase activity between the two was detected using a luciferase reporter; C: RNA immunoprecipitation was used to detect the binding relationship between the two; D and E: Si-hsa_circRNA_102415 was transfected into AGS cells, and the expression level of WNT2B protein in the cells was subsequently measured using Western blotting; F: Transfect plasmid DNA-hsa_ircRNA_102415 into AGS cells, and then use Western blotting to measure the expression level of WNT2B protein in the cells; G: The correlation between the two was evaluated using Pearson correlation test; H: Pearson correlation test was used to evaluate the relationship between the two. Data were reported using mean ± SEM. ^bP < 0.01. circRNA: Circular RNA; miR: MicroRNA; NC: Negative control; si-NC: Small interfering- negative control; pcDNA: Plasmid DNA; MUT: Mutant; WT: Wild-type; RIP: RNA immunoprecipitation.