Circular RNA contributes to gastric cancer by targeting Wnt family member 2B as a competing endogenous RNA

Figure 4 Hsa_circular-RNA_102415 accelerates malignant behaviors of gastric cancer cells by targeted inhibition of microRNA-4529-5p. Transfected microRNA (miR)-RNA-4529-5p mimic and plasmid DNA (pcDNA)-hsa-ccircRNA_102415 into AG cells. Transfected small interferin-hsa_circular-RNA_102415 and control group into BGC823 and AGS cells, with transfection time of 24 hours. A: The cell proliferation level caused by pcDNA-hsa_circular-RNA_102415 and other transfected cells was detected using cell counting kit-8; B and C: The level of cell invasion caused by pcDNA-hsa_ircRNA_102415 after cell transfection was detected using the Transwell method; D: The proportion of different stages of the cell cycle caused by cell transfection of pcDNA-hsa_ircRNA_102415 was detected using flow cytometry; E and F: Detect the level of cell apoptosis caused by pcDNA-hsa_ircRNA_102415 and other transfected cells using flow cytometry. ^bP < 0.01. circRNA: Circular RNA; miR: MicroRNA; NC: Negative control; NC: Negative control; pcDNA: Plasmid DNA; FITC: Fluorescein isothiocyanate.