Circular RNA contributes to gastric cancer by targeting Wnt family member 2B as a competing endogenous RNA

Figure 3 Hsa_circular-RNA_102415 is a microRNA-4529-5p sponge. A: Predicted the binding sites of microRNA (miR)-4529-3p and hsa_circular-RNA_102415 using databases such as circBank; B: The relative luciferase activity was detected using luciferase reporter gene analysis; C: The induction of gene enrichment in AGS cells by negative control (NC) probe and biotinylated miR-497-5p was detected using quantitative real-time polymerase chain reaction (RT-qPCR). Regarding the biological probe NC compared to D; D: Measured RNA binding protein immunoprecipitation using normal mouse IgG, cell lysate, and other inputs. Used RT-qPCR to detect the relative expression levels of two genes in AGS cells; E: Used RT-qPCR to detect the overexpression efficiency of miR-4529-3p mimetics in AGS cells; F: Used RT-qPCR to detect the expression level of hsa_circular-RN_102451 in transfected AGS cells; G: Used RT-qPCR to detect the expression level of miR-497-5p in transfected AGS cells; H: Used RT-qPCR to detect the relative expression levels of miR-4529-5p in human gastric mucosal cell lines and gastric cancer cells. Compared to GES-1. Data were reported using mean ± SEM. n = 3. ^bP < 0.01. circRNA: Circular RNA; miR: MicroRNA; MUT: Mutant; WT: Wild-type; NC: Negative control; Ago2: Argonaute 2; si-NC: Small interfering negative control; pcDNA: Plasmid DNA.