SEL1L-mediated endoplasmic reticulum associated degradation inhibition suppresses proliferation and migration in Huh7 hepatocellular carcinoma cells

Figure 5 EXT2 was the main protein affected by SEL1L knockout in Huh7 cells. A: Venn diagram; B: Volcano plot of differentially expressed genes from scramble and SEL1L knockout Huh7 cells; C: A quantitative real-time polymerase chain reaction assay was conducted to assess EXT2 expression in Huh7 cells following SEL1L knockout; D: Protein expression of EXT2 was detected by western blotting; E: Huh7 cells with either scramble or SEL1L knockout were cultured with or without cycloheximide, and then analyzed by western blotting using the specified antibodies. ^aP < 0.05. ^bP < 0.01. NS: Not significant; SCR: Scramble; KO: Knockout; FC: Fold change; GAPDH: Glyceraldehyde-3-phosphate dehydrogenase; CHX: Cycloheximide.