SEL1L-mediated endoplasmic reticulum associated degradation inhibition suppresses proliferation and migration in Huh7 hepatocellular carcinoma cells

Figure 3 SEL1L knockout affected the growth of Huh7 cells. A: Western blot analysis of heavy-chain binding protein (BiP) and inositol-requiring enzyme (IRE)-1α after SEL1L knockout in Huh7 cells; B: Quantitative real-time polymerase chain reaction was conducted to assess BiP expression and IRE-1α expression; C: The proliferation of scramble and SEL1L knockout Huh7 cells was assessed using the cell counting kit-8; D: The proliferation of scramble and SEL1L knockout Huh7 cells was assessed using the 5-Ethynyl-2’-deoxyuridine; E: The apoptosis rate in scramble and SEL1L knockout Huh7 cells was assessed using annexin V-fluorescein isothiocyanate/7-aminoactinomycin D staining; F: The proliferation of scramble and SEL1L knockout Huh7 cells was assessed using the clone formation assays; G: The migration ability of SEL1L knockout Huh7 cells was detected by the transwell assay. ^aP < 0.05. ^bP < 0.01. ^cP < 0.001. ^dP < 0.0001. GAPDH: Glyceraldehyde-3-phosphate dehydrogenase; BiP: Heavy-chain binding protein; IRE-1α: Inositol-requiring enzyme-1α; KO: Knockout; 7AAD: 7-aminoactinomycin D staining; V-FITC: V-fluorescein isothiocyanate.