Uridine diphosphate glucuronosyltransferase 1A1 prevents the progression of liver injury

Figure 7 Knocking out uridine diphosphate glucuronosyltransferase 1A1 worsened concanavalin A-induced liver injury in mice. A: The enzyme rate method was used to detect the serum level of alanine transaminase in mice; B: The diazo method was used to detect the serum level of total bilirubin and indirect bilirubin; C and E: Pathological analysis of liver tissue by hematoxylin and eosin staining; D and E: The terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate-nick end labelling assay was used to measure hepatocyte apoptosis; F: Western blotting was used to detect the protein levels of uridine diphosphate glucuronosyltransferase 1A1 and cleaved caspase-3; G: Western blotting was used to detect the protein levels of phosphorylated mixed lineage kinase domain-like pseudokinase. ^dP < 0.01 vs the concanavalin A group. UGT1A1: Uridine diphosphate glucuronosyltransferase 1A1; ALT: Alanine transaminase; TBil: Total bilirubin; IBil: Indirect bilirubin; H&E: Hematoxylin and eosin; TUNEL: Transferase-mediated deoxyuridine triphosphate-nick end labelling; p-MLKL: Phosphorylated mixed lineage kinase domain-like pseudokinase; CCl₄: Carbon tetrachloride; KO: Knockout; GAPDH: Glyceraldehyde 3 phosphate dehydrogenase.