Uridine diphosphate glucuronosyltransferase 1A1 prevents the progression of liver injury

Figure 6 Knocking out uridine diphosphate glucuronosyltransferase 1A1 worsened carbon tetrachloride-induced liver injury in mice. A: Western blotting was utilized to screen for the specific Ugt1a1 sgRNA that induced Ugt1a1 gene knockout; B and I: The enzyme rate method was used to detect the serum level of alanine transaminase in mice; C and J: The diazo method was used to detect the serum levels of total bilirubin and indirect bilirubin; D, F, K, and M: Pathological analysis of liver tissue by hematoxylin and eosin (H&E) and Masson staining; E, F, L, and M: The terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate-nick end labelling assay was used to measure hepatocyte apoptosis; G and N: Western blotting was used to detect the protein levels of uridine diphosphate glucuronosyltransferase 1A1 and cleaved caspase-3; H and O: Western blotting was used to detect the protein levels of phosphorylated mixed lineage kinase domain-like pseudokinase. ^bP < 0.01 vs the control sgRNA or control group; ^dP < 0.01 vs the carbon tetrachloride group. UGT1A1: Uridine diphosphate glucuronosyltransferase 1A1; ALT: Alanine transaminase; TBil: Total bilirubin; IBil: Indirect bilirubin; H&E: Hematoxylin and eosin; TUNEL: Transferase-mediated deoxyuridine triphosphate-nick end labelling; p-MLKL: Phosphorylated mixed lineage kinase domain-like pseudokinase; CCl₄: Carbon tetrachloride; KO: Knockout; GAPDH: Glyceraldehyde 3 phosphate dehydrogenase.