Metadherin promotes stem cell phenotypes and correlated with immune infiltration in hepatocellular carcinoma

Figure 5 Metadherin downregulation inhibits hepatocellular carcinoma stem cell phenotypes. A: mRNA expression of Metadherin (MTDH), CD133, Nanog, and Oct4 in 97H and Huh7-RNAi; B: In comparison with that of MTDH-LV-RNAi, the protein expression of CD133 and Nanog was elevated in MHCC-97H-NC and Huh7-NC cells; C: Images depicting sphere formation by 97H-RNAi and Huh7-RNAi cells; D: Immunofluorescence images of Nanog (red) in 97H-LV and Huh7-LV samples. DAPI (blue) was used to stain the nuclei. ^aP < 0.05, ^bP < 0.01, ^cP < 0.001. RNAi: Metadherin knockdown group; RNAi-NC: Metadherin knockdown control group.