Metadherin promotes stem cell phenotypes and correlated with immune infiltration in hepatocellular carcinoma

Figure 4 Metadherin overexpression promotes stem cell phenotypes and self- renewal in hepatocellular carcinoma cell lines. A and B: Through quantitative reverse transcription PCR polymerase chain reaction and Western blot, we determined Metadherin expression and stemness markers; C: The typical pictures of sphere formation assays from 97H-overexpression and Huh7-overexpression cells; D: Flow cytometric analysis of CD133⁺ cells in 97H-overexpressing and Huh7-overexpressing cells. ^aP < 0.05, ^bP < 0.01. OE: Metadherin overexpression group; NC: Overexpression control group; MTDH: Metadherin.