Metadherin promotes stem cell phenotypes and correlated with immune infiltration in hepatocellular carcinoma

Figure 3 Metadherin correlates with the stemness properties in hepatocellular carcinoma. A: Correlation between Metadherin (MTDH) and CD133, Nanog, Oct4 in TCGA; B: Through quantitative reverse transcription PCR, the expression levels of MTDH, CD133, Nanog, Oct4 were measured in attached cells and tumor spheres in 97H and Huh7 cell lines. In tumor spheres, all the four genes were expressed at increased levels; C: MTDH, CD133, and Nanog were higher in attached cells than in tumor spheres. Gene GAPDH served as the internal reference. ^aP < 0.05, ^bP < 0.01, ^cP < 0.001, ^dP < 0.0001.CSC: Cancer stem cells; CC: Cancer cells.