Milk fat globule epidermal growth factor 8 alleviates liver injury in severe acute pancreatitis by restoring autophagy flux and inhibiting ferroptosis in hepatocytes

Figure 3 Milk fat globule epidermal growth factor 8 deficiency aggravated hepatic autophagy and ferroptosis in experimental severe acute pancreatitis. In mice, severe acute pancreatitis was induced by 2 hly intraperitoneal injections of 4.0 g/kg L-arginine. The animals were sacrificed at 72 h after the first injection of L-arginine. Blood and tissue samples were collected. A: Autophagy related protein 16 like protein 1 (ATG16L1) assists LC3 in the formation of autophagosomes; B-E: Western blot analysis and quantitative of the expression of ATG16L1, P62, LC3, prostaglandin-endoperoxide synthase 2 and glutathione peroxidase 4 in the liver; F: Malondialdehyde levels in the liver; G: Glutathione levels in the liver; H: Representative images of DHE staining in the pancreas. n = 3-6/group, error bars indicate the SEM; ^aP < 0.05 vs sham group; ^bP < 0.05 vs vehicle group. SAP: Severe acute pancreatitis; KO: Knockout; WT: Wild type; ATG16L1: Autophagy related protein 16 like protein 1; PTGS2: Prostaglandin-endoperoxide synthase 2; GPX4: Glutathione peroxidase 4; MDA: Malondialdehyde; GSH: Glutathione.